Introduction

Methylene Blue 10X concentrate is used for staining acidoresistant bacteria according to Ziehl-Neelsen, utilizing the dye’s property to stain almost all the bacterial cell’s elements in a mildly alkaline medium. This does not apply to acid-fast bacteria – they remain stained in their primary color. Dilute Methylene Blue 10x concentrate before use. Methylene Blue dye indicates metachromasia and it is suitable for staining strains such as Corynebacterium, Gonococcus, Lactobacillus and for visualizing Pasteurella strain, as well as staining bacterial nuclei and cytoplasm.