Introduction

This kit contains reagents for cytochemical diagnosis of leukemia using bone marrow or whole blood smears.
The method is based on the capability of one type of cell esterase to hydrolyze naphthol AD-D chloroacetate and the capability of another type of cell esterase to hydrolyze 1-naphthyl acetate. Both reactions create free naphthols that bind to various diazonium salts to give a non-soluble precipitates at the reaction site.
The kit is intended for individual testing of horizontally placed sections and it contains reagents for at least 50 tests for detecting specific and non-specific esterase activity in leukocytes. The reagents are applied by dripping until the entire section is covered (1-2 mL).