Introduction
LeukoGnost MPO kit contains reagents for cytochemical diagnosis of leukemia using bone marrow or whole blood smears.
The staining method is based on ability of cellular myeloperoxidase to catalyze hydrogen peroxide reduction, creating water and oxygen that oxidizes 4-chloro-1-naphthol, what in turn forms dark blue to black precipitates at the spot of active peroxidase.
The kit is intended for individual testing of horizontally placed slides and it contains reagents for at least 100 tests for detecting myeloperoxidase activity in leukocytes. The reagents are applied by dripping until the entire slide is covered (1-2 mL).