Introduction

Ladewig reagent is a component of Ladewig Trichrome kit used for staining histology samples with emphasis on differential counterstaining of muscle and collagen tissues. Compared to other BioGnost’s Trichrome kits (Masson and Mallory), the staining procedure is shorter and simpler because of a single incubation in the Ladewig reagent that stains all the necessary structures simultaneously. Ladewig reagent contains Aniline Blue that binds to collagen providing a characteristic blue staining, Acid Fuchsin that dyes the contrast structures red, and Orange G that stains erythrocytes.