Introduction

Ready-To-Use kit for staining frozen and paraffin histology sections using HE (hematoxylin-eosin) method enables complete tissue processing. In case of processing frozen sections, it is completed in just a few minutes after sectioning the sample using cryotome. The staining procedure is simplified and conducted in a few minutes. The kit contains all the reagents necessary for sample processing – BioFix medium for fixing cryostat samples, BioClear New (xylene substitute) for deparaffnation and clearing paraffin sections, alcoholic solutions for rehydration and dehydration of tissue, deionized water, as well as hematoxylin and eosin, and nuclear bluing medium. It also contains mounting medium of very low viscosity and optimal refractive index (BioMount New) in practical packaging. The reagents are placed in practical jars and sections may be directly immersed. They are placed in the order of use in the box, which lowers the possibility of contamination of reagents during staining. Reagents that have not been used during staining frozen or paraffin sections may be additionally used in the following staining procedures. The kit contains one additional jar of BioFix solution used after staining 50 sections. The kit is sufficient for staining approximately 100 sections.