Introduction

BioGnost’s Hematoxylin G3 is a high stability reagent and one of formulations of hematoxylin used in histopathology and cytology, as well as contrasting dye in immunohistochemistry for a more precise nuclear cell staining. Unlike other hematoxylin formulations, hematoxylin acc. to Gill dyes goblet cells in the small intestine epithelium and the respiratory epithelium of the respiratory tract. Compared to BioGnost’s Hematoxylin G1 and G2, Hematoxylin G3 stains the sections with stronger intensity and time needed for getting the results of staining is shorter. Hematoxylin is extracted from logwood (Haematoxylon campechianum L.). Hematoxylin oxidates to hematein and binds with metal ions (mordants), hematein turns into irreplaceable nuclear dye. Positively charged hematein-mordant complex then binds with negatively charged phosphate ions of the DNA’s nucleus, creating characteristic blue coloration. Hematoxylin acc. to GIll is a specific hematoxylin solution used for staining goblet cells and chromatins of both normal and abnormal cross section tissues or cytological smears. BioGnost’s Hematoxylin G1, G2 and G3 are half-oxidized, stabilized with glycols and contain aluminum ions. They stain nuclear membrane, nucleoplasm and nucleolus exceptionally well.