Introduction
BioGnost’s Hematoxylin G2 is a high stability reagent and one of formulations of hematoxylin used in histopathology and cytology for a more precise nuclear cell staining. Compared to Hematoxylin G1, Hematoxylin G2 stains preparations with greater intensity. That results in shorter waiting periods. Hematoxylin G2 is ideal for darker, more intense staining of cellular nuclei of cytological smears or histological preparations, although it is also often used for contrast staining in immunohistochemistry. Unlike other hematoxylin formulations, hematoxylin acc. to Gill dyes goblet cells in the small intestine epithelium and the respiratory epithelium of the respiratory tract. Hematoxylin is extracted from logwood (Haematoxylon campechianum L.). Hematoxylin oxidates to hematein and binds with metal ions (mordants), hematein turns into irreplaceable nuclear dye. Positively charged hematein-mordant complex then binds with negatively charged phosphate ions of the DNA’s nucleus, creating characteristic blue coloration. Hematoxylin acc. to GIll is a specific hematoxylin solution used for staining goblet cells and chromatins of both normal and abnormal cross section tissues or cytological smears. BioGnost’s Hematoxylin G1, G2 and G3 are half-oxidized, stabilized with glycols and contain aluminum ions. They stain nuclear membrane, nucleoplasm and nucleolus exceptionally well.