Introduction

Paraffin is the most commonly used medium for infiltration, embedding and creating tissue blocks. It enables the processing of large amounts of tissue samples in a short period of time, serial samples can be easily obtained. Routine staining, but also the most complicated ones can be done simply. BioGnost’s BioWax Plus 56/58 paraffin is an optimal blend of paraffin wax and plastic polymers without added dimethyl sulfoxide (DMSO). Its main characteristics are low-level shrinking during cooling and ideal properties during cutting that provide optimal results during strips formation. Enables excellent infiltration into tissues and reduces the tendency of tissue to crack. Its granulated form allows easier handling and faster melting with a melting point at 54°C/57°C. It is used for permeating tissue and forming paraffin blocks that enable creating paraffin sections. Sections can then be stained using standard histological techniques or use in different immunohistochemical and molecular biological reactions.